hHSP70 gene transfection on rat coronary artery myocardial ischemia-reperfusion injury in rats
【Abstract】 Objective: To study the hHSP70 gene transfection through the coronary artery on myocardial ischemia-reperfusion injury effects, and to explore its possible mechanism. Click below to download the original:
/ paperimg/soft/I194.doc
Methods: (1) Construction of replication-defective recombinant adenovirus: Using molecular cloning obtained by means of carrying enhanced green fluorescent protein (EGFP) recombinant plasmids carrying human heat shock protein 70 recombinant plasmid to cells by homologous recombination Constructing the replication-defective recombinant adenovirus Adv-EGFP, Adv-hHsp70, were amplified in 293 cells, the application cesium chloride density gradient centrifugation purified virus. Titer purified by cesium chloride, respectively, Adv-EGFP: 2.0
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