1.6-diphosphate on cerebral ischemia-reperfusion injury of apoptotic cells and ref-1.p38 Research
【Abstract】 Objective To investigate the 1,6-diphosphate (FDP) on cerebral ischemia / reperfusion (I / R) injury and its mechanism. Methods 90 male SD rats were randomly divided into three groups: sham operation control group (C group), cerebral ischemia group (I group), fructose group (F group) 30. I group and F group rats down in the condensate to promote vein 24 hours after bilateral occlusion of the bilateral common carotid artery 5 minutes after the re-opening production of cerebral ischemia model, C group only exposed to non-condensable and non-breaking cone artery occlusion common carotid artery, F group and then the start of intravenous infusion of 1,6 - diphosphate 1.5ml/kg, I group and C group were intravenous injection of normal saline 1.5ml/kg. Groups after reperfusion 2h, 6h, 12h, 24h, 48h, 72h time to take samples and serum SOD, MDA content, and preparation of brain tissue biopsy, using immunohistochemical staining method and TUNEL in situ cell apoptosis detection method Determination of P38 protein and Ref-1 protein expression and apoptotic cell number. The results compared with the C group, I group each time point after reperfusion significantly reduced SOD activity, MDA content increased significantly (P
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